The polymerase chain reaction (PCR) and molecular hybridization were used for detecting and typing human papillomavirus (HPV) in 40 patients with clinical lesions as a control group and in 20 healthy subjects, biopsies on clinically normal oral mucosa. Amplification of HPV DNA was performed using specific oligonucleotide primers: MY09 and MY11 and for typing hybridization was used in plates comprising a mixture of probes of high, intermediate and low oncogenic risk, such as types 2, 4, 6, 11, 13, 16, 18 and 32. The results demonstrated the presence of HPV viral genome in 55 percent (22/40) of benign lesions of the mouth and 10 percent (2/20) in the control group. In oral benign lesions that were positive by PCR, we observed 90.9 percent (20/22) of the type HPV low risk and 9.1 percent (2/22) of HPV and low oncogenic risk lato. In the control group it was detected 5 percent (1/20) of low-risk HPV and 5 percent (1/20) of HPV high and low oncogenic risk.
Substance P is the first neuroactive substance that is proposed as a neurotransmitter. As for the pharmacological properties of substance P, they were demonstrated to check exhibiting potent hypotensive properties that were not blocked by atropine. He was later identified as the first active neuropeptide and was proposed as a neurotransmitter. In the 70s the structure is now known to contain eleven amino acids, of which six corresponding to the C-terminus are essential for its biological activity was determined. Substance P (SP) is a neuropeptide of eleven amino acids, are much smaller than other neurotransmitters amounts, is synthesized in the cell body and transported along the axon to the terminals, it is synthesized as part of a prepropeptide ( preproteins or precursors) of higher molecular weight which then moves to the cisterns of the endoplasmic reticulum, where it is disintegrated to produce a proprotein. The proprotein is transported to the Golgi apparatus, where they may fac...
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