The polymerase chain reaction (PCR) and molecular hybridization were used for detecting and typing human papillomavirus (HPV) in 40 patients with clinical lesions as a control group and in 20 healthy subjects, biopsies on clinically normal oral mucosa. Amplification of HPV DNA was performed using specific oligonucleotide primers: MY09 and MY11 and for typing hybridization was used in plates comprising a mixture of probes of high, intermediate and low oncogenic risk, such as types 2, 4, 6, 11, 13, 16, 18 and 32. The results demonstrated the presence of HPV viral genome in 55 percent (22/40) of benign lesions of the mouth and 10 percent (2/20) in the control group. In oral benign lesions that were positive by PCR, we observed 90.9 percent (20/22) of the type HPV low risk and 9.1 percent (2/22) of HPV and low oncogenic risk lato. In the control group it was detected 5 percent (1/20) of low-risk HPV and 5 percent (1/20) of HPV high and low oncogenic risk.
El cráneo contiene tres elementos : tejido nervioso, sangre y liquido cefalorraquídeo, cuyo volumen total siempre debe ser constante. The skull contains three elements: nervous tissue , blood and cerebrospinal fluid , the total volume should always be constant. Череп содержит три элемента: нервной ткани , крови и цереброспинальной жидкости , общий объем должен быть всегда постоянным. 頭骨包含三個元素：神經組織，血液和腦脊髓液，庫約общая總是Дебесерконстант量。
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